Comparison of the genomic expression of mice fed benaGene or Calorie Restricted as compared to Control Mice

Calorie Restricted Mice were fed 40% less than baseline ad librium. benaGene supplemented mice and Control group mice were allowed unrestricted feed. Of the 363 genes that showed increases or decreases in expression that were common to both the Calorie Restricted and benaGene fed mice, 356 of these genes (98.1%) moved in the same direction away from the control group mice, indicating that feeding benaGene to mice mimics a similar genomic expression as is seen in mice that are Calorie Restricted for the genes that are expressed in common. Effective doses of benaGene fed to Canorhabitis elegans (worms), Drosophila melanogaster (flys) and C57Bl/6 mice increase lifespan by 20 to 40% by increasing the levels of favorable proteins and decreasing detrimental proteins expressed by the DNA gene sequences.

Some of the Genes upregulated and down regulated by calorie restriction (CR) as compared to the control group, and benaGene supplemented mice as compared to the control group (mouse liver tissue data) include:

Symbol	Gene Name	CR to C	benaGene to C
Acp1	acid phosphatase 1, soluble	-1.1	-0.9
Adn	adipsin	-1.1	-0.7
Apoa4	apolipoprotein A-IV	-0.7	-0.8
Apoa4	apolipoprotein A-IV	-0.7	-1
Asb13	ankyrin repeat and SOCS box-containing protein 13	-0.9	-0.7
Bpnt1	bisphosphate 3'-nucleotidase 1	-0.6	-0.7
Brp17	brain protein 17	-1.1	-0.4
Btf3	basic transcription factor 3	-0.4	-0.5
Cd36	CD36 antigen	-1.2	-0.7
Cd36	CD36 antigen	-1.3	-0.8
Cml5	camello-like 5	-1.3	-0.7
Cnbp	cellular nucleic acid binding protein	-0.9	-0.5
Col3a1	procollagen, type III, alpha 1	-1.1	-0.7
Cryz	crystallin, zeta	-0.7	-0.7
Cugbp2	CUG triplet repeat,RNA binding protein 2	-0.5	-0.9
Cyp2b9	cytochrome P450, family 2, subfamily b, polypeptide 9	-2.5	-1
Cyp7a1	cytochrome P450, family 7, subfamily a, polypeptide 1	1.6	0.6
Desrt	developmentally and sexually retarded with transient immune abnormalities	0.9	0.7
Desrt	developmentally and sexually retarded with transient immune abnormalities	1.3	0.6
Dgat2l1	diacylglycerol O-acyltransferase 2-like 1	-1.8	-1.4
Dmd	dystrophin, muscular dystrophy	-1	-0.6
Dusp1	dual specificity phosphatase 1	-0.5	-1
Egfl5	EGF-like-domain, multiple 5	-0.8	-0.7
Egfr	epidermal growth factor receptor	1.1	0.5
Elovl6	ELOVL family member 6, elongation of long chain fatty acids (yeast)	-1.2	-0.6
Elovl6	ELOVL family member 6, elongation of long chain fatty acids (yeast)	-1.3	-0.6
Fabp4	fatty acid binding protein 4, adipocyte	-1.4	-0.8
Fabp4	fatty acid binding protein 4, adipocyte	-1.7	-1.4
Fabp5	fatty acid binding protein 5, epidermal	0.8	1.4
Fabp5	fatty acid binding protein 5, epidermal	1.2	1.8
Fbxo17	F-box only protein 17	0.9	0.8
Fin15	fibroblast growth factor inducible 15	-0.7	-0.6
Foxa3	forkhead box A3	1	0.7
Foxq1	forkhead box Q1	1.1	2.1
Foxq1	forkhead box Q1	1.9	2.2
Fsp27	fat specific gene 27	-1	-0.9
G0s2	G0/G1 switch gene 2	-0.6	-0.5
Gas2	growth arrest specific 2	-0.7	-0.6
Gdf15	growth differentiation factor 15	-1.3	-0.6
Gpam	glycerol-3-phosphate acyltransferase, mitochondrial	-1	-0.6
Gpd1	glycerol-3-phosphate dehydrogenase 1 (soluble)	-0.5	-0.6
Gsta2	glutathione S-transferase, alpha 2 (Yc2)	-0.6	-1
Gstm3	glutathione S-transferase, mu 3	-1.3	-1.1
Gstm3	glutathione S-transferase, mu 3	-1	-1.1
Hao3	hydroxyacid oxidase (glycolate oxidase) 3	-0.9	-0.4
Hnmt	histamine N-methyltransferase	-0.7	-0.6
Hnrpr	heterogeneous nuclear ribonucleoprotein R	-0.7	-0.7
Hspb1	heat shock protein 1	-0.9	-0.5
Hspca	heat shock protein 1, alpha	-0.9	-0.6
Ifi205	interferon activated gene 205	-1.2	-1.6
Irf7	interferon regulatory factor 7	-1.6	-0.6
Jun	Jun oncogene	-0.6	-1.3
Laptm4b	lysosomal-associated protein transmembrane 4B	-0.7	-1.1
Lasp1	LIM and SH3 protein 1	-0.9	-0.9
Lcn2	lipocalin 2	-1.8	-0.7
Lgals1	lectin, galactose binding, soluble 1	-1.6	-0.6
Luc7l2	LUC7-like 2 (S. cerevisiae)	-1.1	-0.6
Ly6a	lymphocyte antigen 6 complex, locus A	-1.6	-0.8
Ly6d	lymphocyte antigen 6 complex, locus D	-3.4	-2.2
Map3k5	mitogen activated protein kinase kinase kinase 5	1.2	0.7
Mme	membrane metallo endopeptidase	-1.1	-0.7
Oas1g	2'-5' oligoadenylate synthetase 1G	-1.2	-1.2
Olig1	oligodendrocyte transcription factor 1	-0.8	-0.5
Omd	osteomodulin	-0.8	-0.6
Orm2	orosomucoid 2	-1.2	-0.6
Osbpl3	oxysterol binding protein-like 3	-0.9	-1.1
Osp94	osmotic stress protein	-1.2	-0.6
Pbx2	pre B-cell leukemia transcription factor 2	1.3	1.5
Phlda1	pleckstrin homology-like domain, family A, member 1	1.3	1.2
Plscr2	phospholipid scramblase 2	-0.9	-1.1
Ppicap	peptidylprolyl isomerase C-associated protein	-1.1	-0.5
Rbms1	RNA binding motif, single stranded interacting protein 1	0.8	0.9
Rgs5	regulator of G-protein signaling 5	-1.3	-0.6
7-Sep	septin 7	-0.8	-0.8
Serpina4-ps1	serine (or cysteine) proteinase inhibitor, clade A, member 4, pseudogene 1	3.6	1.8
Serpinb1a	serine (or cysteine) proteinase inhibitor, clade B, member 1a	-1.7	-1.2
Sgk	serum/glucocorticoid regulated kinase	-0.7	-0.4
Slco1a1	solute carrier organic anion transporter family, member 1a1	1	0.6
Snap23	synaptosomal-associated protein 23	-0.9	-0.6
Socs2	suppressor of cytokine signaling 2	2.8	0.8
Sycp3	synaptonemal complex protein 3	-1.3	-1.1
Tubb2	tubulin, beta 2	-2.7	-1
Usp18	ubiquitin specific protease 18	-1.8	-0.8
Vnn1	vanin 1	-1.4	-0.8
Zfp207	zinc finger protein 207	-1.1	-0.5